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| Instrument Configurations |
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P/N 6200 : 2-Plex (Green, Yellow)
P/N 62H0 : 2-Plex (Green, Yellow)+HRM (High Resolution Melt)
P/N 6500 : 5-Plex (Green, Yellow, Orange, Red, Crimson)
P/N 65H0 : 5-Plex (Green, Yellow, Orange, Red, Crimson)+HRM (High Resoultion Melt)
P/N 6600 : 6-Plex (Blue, Green, Yellow, Orange, Red, Crimson) |
Rotor Configurations |
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30 µL x 100-wells: GeneDisc™ 100 (heat-sealed plate) AVAILABLE SOON
0.1 mL x 72-wells: GeneDisc™ 72 (heat-sealed plate)
0.1 mL x 72-wells: 0.1 mL strip tubes (strips of 4, matching caps)
0.2 mL x 36-wells: 0.2 mL tube (attached cap, domed or flat) |
Channel Information |
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Channel |
Excite |
Detect |
Some Example Fluorophores Detected |
| Blue |
365±20 |
460±15 |
Biosearch Blue™, Marina Blue®, Bothell Blue®, Alexa Fluor® 350 |
| Green |
470±10
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510±5
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FAM™, SYBR® Green 1, Fluorescein, EvaGreen™, Alexa Fluor® 488 |
| Yellow |
530±5 |
555±5 |
JOE™, VIC™, HEX™, TET™, CAL Fluor® Gold 540, Yakima Yellow® |
| Orange |
585±5 |
610±5 |
ROX™, CAL Fluor® Red 610, Cy3.5™, Texas Red®, Alexa Fluor®568 |
| Red |
625±10 |
660±10 |
Cy5™, Quasar™ 670, LightCycler Red640®, Alexa Fluor 633
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| Crimson |
680±5 |
>712(LP) |
Quasar™ 705, LightCycler Red705®, Alexa Fluor® 680
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| HRM |
460±15 |
510±5 |
SYTO®9, LC Green®, LC Green® Plus+, EvaGreen™... |
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Thermal Performance |
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Uniformity: ± 0.01°C
Resolution: ± 0.02°C
Range: ambient–99°C
Maximum Ramp Rate: 10°C/sec (air) |
Optical System |
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Fixed path length
Separate color high-intensity LED (light-emitting diode) excitation source per channel
Separate emission filter per channel |
Detector |
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Photomultiplier (PMT) detector with variable or automatic gain setting (sensitivity control), user selected |
Dimensions |
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370mm (14.6") wide, 420mm (16.5") deep, 560mm (22") deep door open, 275mm (10.8") high |
Weight |
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14 kg (31 lbs) |
| Electrical |
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100-120VAC @ 60Hz, 200-240VAC @ 50Hz
Power consumption: 8VA (idle/standby), 560VA (peak) upgrades |
| Minimum Computer |
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Pentium™ IV, 2.6GHz (Desktop), Pentium M, 1.6GHz (Laptop) or equivalents
256Mb RAM, Windows XP®, USB or RS232 Serial Port connection |
| Software |
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Extensive analysis, graphing and statistical functions built-in. Unlimited use software licence included, free upgrades (by web download), Windows XP, Pentium IV (2GHz) or higher PC, USB or Serial Port required |
40 Cycle Run Time |
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Typically 40 minutes to 1.5 hours (rotor and protocol dependant) |
Supported Volumes |
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5 µL to 100 µL (rotor and protocol dependant)
Typical reaction volume 20 µL |
Instrument Color |
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Outback Red or Bondi Blue |
HRM (High Resolution Melt) |
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New HRM capability is optional and comprises a dedicated high-performance optical subsystem, additional hardware and specific HRM analysis software (fully integrated). Thermal uniformity ±0.01°C, Resolution ±0.02°C, Range ambient–99°C. HRM data acquisition (read) rate of 20 reads for each 0.02°C increment (=1000 reads/°C)
NOTE: Conventional DNA Melt/Anneal capability and analysis software (including auto-call genotyping capability) is standard on all models |
| DNA Concentration Measurement |
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For direct measurement of nucleic acid concentration using fluorescent dyes (e.g. PicoGreen®, RiboGreen®, etc). Comprises dedicated analysis software (standard on all models) |
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