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Applications  
 
 
  The NanoDrop® ND-1000 Spectrophotometer can be used to measure protein concentration via the Modified Lowry Protein Assay. This method is based on the widely cited Lowry assay and involves the reaction of protein with cupric sulfate in alkaline solution, resulting in the formation of tetradentate copper-protein complexes. The Folin-Ciocalteu Reagent is effectively reduced in proportion to the chelated copper-complexes resulting in a water-soluble blue product measured at 650 nm.  
 


 
 
Key Features

Both Regular and Mini Bradford assays can be run
Allows generation of standard curves with up to 7 standards. Four curve fitting analysis options available
Concentration of protein is calculated and displayed
Reblank feature allows for the re-analysis of previous sample with a new blank
Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
Sample ID field is key-focused for easy bar coder use
Absorbance is represented as a 1mm path equivalent

Measurement Concentration Range


Assay Type
Detection Limit (ug/ml)
Approximate Upper Limit (ug/ml)
Typical Reproducibility
(minimum 5 replicates)
(SD= ug/ml; CV= %)
Regular BCA
100
8000
sample range 100-500 ug/ml: ± 25 ug/ml
sample range 500-8000 ug/ml: ± 5%

Mini Bradford

15
125
sample range 15-50 ug/ml: ± 4 ug/ml
sample range 50-125 ug/ml: ± 5%



 

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