JH BIO Innovations - Distributors of Scientific Instrumentation

The NanoDrop® ND-1000 Spectrophotometer can be used to measure protein concentration via the BCA (Bicinchoninic Acid) Protein Assay. This assay is often used for more dilute protein solutions and/or for proteins in the presence of components that also have significant UV (280 nm) absorbance. The resulting Cu-BCA chelate formed in the presence of protein is measured at its at its wavelength maximum of 562 nm. The BCA Assay requires that a standard curve be generated before unknown proteins can be measured.

Both Regular and Mini BCA assays can be run

Allows generation of standard curves with up to 7 standards. Four curve fitting analysis options available

Concentration of protein is calculated and displayed

Reblank feature allows for the re-analysis of previous sample with a new blank

Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature

Sample ID field is key-focused for easy bar coder use

Detection Limit (mg/ml)

Approximate Upper Limit (mg/ml)

Typical Reproducibility
(minimum 5 replicates)
(SD= mg/ml; CV= %)

± 2% (over entire range)

± 0.01 mg/ml (over entire range)