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Applications
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The NanoDrop® ND-1000 Spectrophotometer will accurately and reproducibly measure purified protein samples up to 100 mg/ml (BSA), without dilution. To do this, the instrument detects the high concentration and automatically utilizes the 0.2mm pathlength to calculate the absorbance.
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Key Features
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Six different reference types available : 1 abs=1mg/ml, BSA, IgG, Lysozyme, user entered mass extinction coefficient, user entered molar extinction coefficient |
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Concentration of protein is calculated and displayed as mg/ml. |
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Reblank feature allows for the re-analysis of previous sample with a new blank |
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Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature |
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260/280 ratio calculated and displayed |
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Sample ID field is key-focused for easy bar coder use |
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Absorbance is represented as a 1mm path equivalent |
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Measurement Concentration Range
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Detection Limit
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Approximate Upper Limit (pmol/ul)
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Typical Reproducibility
(minimum 5 replicates)
(SD= pmol/ul; CV= %) |
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0.10 mg/ul |
100 mg/ul |
sample range 0.05-10 mg/ml: ± 0.10 mg/ml
sample range >10 mg/ml: ± 2% |
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