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Applications
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NanoDrop® ND-3300 Fluorospectrometer
The NanoDrop® ND-3300 Fluorospectrometer is a multi-source optical instrument for measuring fluorescence. This space-saving fluorometer offers exclusive features that allow investigators the flexibility to use fluorometry in valuable new ways
The uniquely clean optics of the patented retention system, combined with
virtual filtering (see below) for the white LED applications, enables
measurements across a wide range of wavelengths using sample volumes of 1-2 ul
without cuvettes and costly filter changes
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The excitation source comes from one of the three solid-state LEDs housed in a block adjacent to the measurement pedestal (see below for more details about the LEDs). Emitted light is collected using the traditional 90° angle from the respective excitation source. A 2048-element CCD array detector, covering 400-750nm, is connected by an optical fiber to the optical measurement surface. The spectrometer is configured with a cut filter to eliminate light transmission below 395 nm.
An added convenience is that no external power supply is required. All of the operating power is supplied by the USB port on the PC.
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Key Features
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Includes analysis capability for dsDNA, RNA and "other" (analysis constant selectable between 15-55). |
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High absorbance capability: up to 3700 ng/ul for dsDNA . |
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Reblank feature allows new for the re-analysis of previous sample with a new blank |
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Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature. |
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260/280 and 260/230 ratios calculated and displayed. |
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Sample ID field is key-focused for easy bar coder use. |
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Absorbance is represented as a 10mm path equivalent. |
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Supported Operating Systems |
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Microsoftw Windows 2000 or XP; Software versions prior to V 3.2.0 also can operate onWindows 98 or ME; no Mac version currently available. |
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Required Hardware |
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233 MHz processor with free USB port, CD ROM Drive, 32 MB RAM, 40 MB of Free Hard Drive |
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Optional |
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MS Excel or similar spreadsheet program for manipulating data. |
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