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Applications
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The capability to pre-select viable fluorescent-tagged hybridization probes for gene expression in microarrays can eliminate potentially flawed samples and improve research effectiveness. The NanoDrop software facilitates the measurement of DNA concentration and dye labeling effectiveness. The NanoDrop® ND-1000 Spectrophotometer measures the absorbance of the fluorescent dye, allowing detection at dye concentration as low as 0.2 pmol/ul.
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Key Features
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Includes analysis capability for dsDNA, RNA and "other" (analysis constant selectable between 15-55).
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Measurement of wide variety of fluorescent dyes possible: Cy3, Cy5, Cy3.5, Cy5.5, Alexas 488, 546, 555, 594, 647 and 660 |
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Concentration of nucleic acid and up to two dyes calculated and displayed |
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Reblank feature allows for the re-analysis of previous sample with a new blank |
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Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature |
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260/280 ratio calculated and displayed |
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Sample ID field is key-focused for easy bar coder use |
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Absorbance is represented as a 1mm path equivalent |
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Measurement Concentration Range
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Detection Limit
(pmol/ul) |
Approximate Upper Limit (pmol/ul)
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Typical Reproducibility
(minimum 5 replicates)
(SD= pmol/ul; CV= %) |
Cy3, Cy3.5, Alexa Fluor 555, Alexa Fluor 660
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0.20 |
100 |
sample range 0.20-4.0 pmol/ul: ± 0.20 pmol/ul
sample range >4.0 pmol/ul: ± 2% |
Cy5, Cy5.5 and Alexa Fluor 647 |
0.12 |
60 |
sample range 0.12-2.4 pmol/ul: ± 0.12 pmol/ul
sample range >2.4 pmol/ul: ± 2% |
Alexa Fluor 488 and Alexa Fluor 594 |
0.40 |
215 |
sample range 0.40-8.0 pmol/ul: ± 0.40 pmol/ul
sample range >8.0 pmol/ul: ± 2% |
Alexa Fluor 546 |
0.30 |
145 |
sample range 0.30-6.0 pmol/ul: ± 0.30 pmol/ul
sample range >6.0 pmol/ul: ± 2% |
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